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Genes expressed by the biological control bacterium P seudomonas protegens Pf ‐5 on seed surfaces under the control of the global regulators GacA and RpoS
Author(s) -
Kidarsa Teresa A.,
Shaffer Brenda T.,
Goebel Neal C.,
Roberts Daniel P.,
Buyer Jeffrey S.,
Johnson Aaron,
Kobayashi Donald Y.,
Zabriskie T. Mark,
Paulsen Ian,
Loper Joyce E.
Publication year - 2013
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12066
Subject(s) - rpos , biology , gene , mutant , gene expression , genetics , transcriptome , promoter
Summary Gene expression profiles of the biological control strain P seudomonas protegens Pf ‐5 inhabiting pea seed surfaces were revealed using a whole‐genome oligonucleotide microarray. We identified genes expressed by Pf ‐5 under the control of two global regulators ( GacA and RpoS ) known to influence biological control and secondary metabolism. Transcript levels of 897 genes, including many with unknown functions as well as those for biofilm formation, cyclic diguanylate (c‐di‐ GMP ) signalling, iron homeostasis and secondary metabolism, were influenced by one or both regulators, providing evidence for expression of these genes by Pf ‐5 on seed surfaces. Comparison of the GacA and RpoS transcriptomes defined for Pf ‐5 grown on seed versus in broth culture overlapped, but most genes were regulated by GacA or RpoS under only one condition, likely due to differing levels of expression in the two conditions. We quantified secondary metabolites produced by Pf ‐5 and gacA and rpoS mutants on seed and in culture, and found that production profiles corresponded generally with biosynthetic gene expression profiles. Future studies evaluating biological control mechanisms can now focus on genes expressed by Pf ‐5 on seed surfaces, the habitat where the bacterium interacts with seed‐infecting pathogens to suppress seedling diseases.