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Concentration of osmoregulated periplasmic glucans ( OPGs ) modulates the activation level of the RcsCD RcsB phosphorelay in the phytopathogen bacteria D ickeya dadantii
Author(s) -
BontempsGallo Sébastien,
Madec Edwige,
Dondeyne Jacqueline,
Delrue Brigitte,
RobbeMasselot Catherine,
Vidal Olivier,
Prouvost AnneFrance,
Boussemart Gilles,
Bohin JeanPierre,
Lacroix JeanMarie
Publication year - 2013
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12054
Subject(s) - periplasmic space , protein data bank (rcsb pdb) , virulence , biology , biochemistry , bacteria , microbiology and biotechnology , escherichia coli , gene , genetics
Summary Osmoregulated periplasmic glucans ( OPGs ) are general constituents of many Proteobacteria. Synthesis of these oligosaccharides is repressed by increased osmolarity of the medium. OPGs are important factors required for full virulence in many zoo‐ or phytopathogens including D ickeya dadantii . The phytopathogen enterobacterium D . dadantii causes soft‐rot disease on a wide range of plant species. The total loss of virulence of opg ‐negative strains of D . dadantii is linked to the constitutive activation of the RcsCD RcsB phosphorelay highlighting relationship between this phosphorelay and OPGs . Here we show that OPGs control the RcsCD RcsB activation in a concentration‐dependent manner, are required for proper activation of this phosphorelay by medium osmolarity, and a high concentration of OPGs in planta is maintained to achieve the low level of activation of the RcsCD RcsB phosphorelay required for full virulence in D . dadantii .