Premium
Development of microsatellite markers for the endangered semi‐shrub Chimaphila umbellata ( E ricaceae)
Author(s) -
Kikuchi Ai,
Miyazaki Taisuke,
Maki Masayuki
Publication year - 2018
Publication title -
plant species biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 36
eISSN - 1442-1984
pISSN - 0913-557X
DOI - 10.1111/1442-1984.12198
Subject(s) - biology , microsatellite , endangered species , loss of heterozygosity , genetic diversity , pyrosequencing , genetics , allele , shrub , evolutionary biology , botany , ecology , gene , population , demography , sociology , habitat
Based on the DNA sequencing reads obtained using 454 pyrosequencing, primers amplifying 16 microsatellite loci were developed for the endangered semi‐shrub Chimaphila umbellata , which occurs sporadically in the Japanese Archipelago. These 16 loci were polymorphic in the populations sampled from the Hokkaido and Tohoku Districts; the mean number of alleles was 3.31 and 3.44, and the mean expected heterozygosity was 0.42 and 0.44, respectively. These loci were not linked to each other and contained no null alleles. Amplification using these primers was also tested in the congeneric species C. japonica , but only three of them successfully amplified DNA of the species. These markers will be used to examine genetic diversity and genetic differentiation in populations of C. umbellata .