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Root endophytic Chaetomium cupreum promotes plant growth and detoxifies aluminum in Miscanthus sinensis A ndersson growing at the acidic mine site
Author(s) -
Haruma Toshikatsu,
Yamaji Keiko,
Masuya Hayato,
Hanyu Kazuyo
Publication year - 2018
Publication title -
plant species biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 36
eISSN - 1442-1984
pISSN - 0913-557X
DOI - 10.1111/1442-1984.12197
Subject(s) - miscanthus sinensis , endodermis , biology , mycelium , camellia sinensis , siderophore , botany , chaetomium , seedling , stele , rhizome , biochemistry , miscanthus , fusarium , ecology , bioenergy , renewable energy , gene
Miscanthus sinensis A ndersson grows naturally at the H itachi mine. The root‐zone soil was acidic and contained high concentrations of C u, P b, Z n and exchangeable A l. Adventitious roots accumulated high concentrations of A l and F e, but not other heavy metals. The purpose of this study was to elucidate the mechanism of tolerance of A l in M. sinensis , focusing on its chemical interaction with root endophytes. We isolated Chaetomium cupreum , which produced siderophores, from adventitious roots of M. sinensis via CAS assay. In inoculation tests, C. cupreum promoted M. sinensis seedling growth and increased A l and F e uptake in the roots, although C. cupreum did not stimulate M. sinensis to produce A l detoxicants, such as citric and malic acids. Observation of the pattern of A l localization in the roots clarified that C. cupreum reduced A l toxicity in M. sinensis via compartmentalizing A l into fungal mycelia surrounding the roots and creating a less toxic A l‐localization pattern, allocating A l to the epidermis, endodermis and stele of roots. In conclusion, our results indicated that C. cupreum increases A l tolerance in M. sinensis growing at the acidic mine site.