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Characterization of new microsatellite loci in Pieris amamioshimensis (Ericaceae), a species nearly extinct in the wild
Author(s) -
Mitsui Yuki,
Nagasawa Junichi,
Maeda Yoshiyuki,
Setoguchi Hiroaki
Publication year - 2018
Publication title -
plant species biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 36
eISSN - 1442-1984
pISSN - 0913-557X
DOI - 10.1111/1442-1984.12193
Subject(s) - biology , microsatellite , loss of heterozygosity , locus (genetics) , genetic diversity , ex situ conservation , genetics , allele , population , inbreeding , endangered species , evolutionary biology , linkage disequilibrium , zoology , ecology , haplotype , gene , demography , sociology , habitat
A set of 11 polymorphic microsatellite markers has been developed and characterized for the critically endangered species Pieris amamioshimensis . Fifty‐nine individuals of an ex‐situ population were used to identify these markers. The total number of alleles for each locus ranged from 3 to 9, with an average of 5.4. The expected heterozygosities ( H S ) and observed heterozygosities ( H O ) ranged from 0.47 to 0.77 and 0.22 to 0.88, respectively. In total, four loci exhibited significant deviations from Hardy–Weinberg equilibrium: two loci showed significant heterozygosity excess and the other two loci showed significant heterozygosity deficit. The polymorphism information content (0.43 ≤ PIC ≤ 0.73), the probability of exclusions (PE 1 = 0.9565, PE 2 = 0.9969 and PE 3 = 0.9999) and probabilities for identity (PI = 3.78 × 10 −9 and PI‐Sib = 2.35 × 10 −4 ) suggest that these markers are useful for estimating not only genetic diversity but also parentage, for the ex‐situ conservation management of populations.