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Development and characterization of 24 microsatellite markers in Primula tosaensis , an endangered primrose, using MiSeq
Author(s) -
Yamamoto Masaya,
Handa Yoshihiro,
Aihara Hiroki,
Setoguchi Hiroaki
Publication year - 2018
Publication title -
plant species biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 36
eISSN - 1442-1984
pISSN - 0913-557X
DOI - 10.1111/1442-1984.12192
Subject(s) - biology , microsatellite , genetic diversity , primulaceae , endangered species , primula , population , conservation genetics , evolutionary biology , genetics , allele , zoology , botany , ecology , gene , demography , sociology , habitat
Primula tosaensis (Primulaceae) is an endangered primrose endemic to Japan. In this study, 24 novel microsatellite markers were developed using Illumina MiSeq sequencing to facilitate conservation of this endangered species. The genetic diversity and polymorphisms of these novel markers were measured in 32 individuals from a wild P. tosaensis population. The number of alleles and expected heterozygosities ranged from 2 to 5 (mean = 2.8) and from 0.119 to 0.724 (mean = 0.395), respectively. All loci were in Hardy–Weinberg equilibrium. The markers developed in this study will provide a powerful and practical tool for investigating the population structure and genetic diversity of P. tosaensis .