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Multidisciplinary approaches for species delimitation in Sisyrinchium ( I ridaceae)
Author(s) -
Fachinetto Juliana,
KaltchukSantos Eliane,
Dellanhese Inácio Camila,
Eggers Lilian,
de SouzaChies Tatiana T.
Publication year - 2018
Publication title -
plant species biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 36
eISSN - 1442-1984
pISSN - 0913-557X
DOI - 10.1111/1442-1984.12181
Subject(s) - biology , selfing , ploidy , reproductive isolation , taxon , evolutionary biology , pollen , botany , pollination , zoology , population , genetics , gene , demography , sociology
A remarkable morphological similarity in natural populations of different groups of Sisyrinchium spp. ( I ridaceae) makes classification of some species difficult. The present paper focused on two morphological categories of S. sellowianum ( MC‐I and MC‐II ) with distinct morphological traits. Morphological, cytogenetic, reproductive biology and genetic studies were performed, aiming to elucidate and better characterize such variation. The basic chromosome number established for the species was x = 9 and diploid and tetraploid populations were observed for MC‐I , whereas MC‐II showed only diploid populations. Different pollen morphologies were recognized in each morphological category. Based on pollination experiments, MC‐I is out‐crossing, whereas MC‐II is selfing. The populations were highly differentiated ( F ST = 0.46, θ B = 0.62). In MC‐I , observed variation was greater within populations (69%) than among populations (31%), whereas in MC‐II , 61% of the variation was among populations and 39% within populations. This study evidenced that the two morphological categories correspond to distinct and reproductively isolated species and that floral traits reflect their breeding system, with allogamous plants in MC‐I and autogamous in MC‐II . Furthermore, MC‐II is unveiled as S. platycaule , a neglected taxon of Sisyrinchium in B razil. The tools applied in this study were substantially able to check the identity of the two morphological categories as two different species and present the variation encountered in the taxa.