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Development and characterization of EST‐SSR markers in Asarum sakawanum var. stellatum and cross‐amplification in related species
Author(s) -
Takahashi Daiki,
Sakaguchi Shota,
Setoguchi Hiroaki
Publication year - 2017
Publication title -
plant species biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 36
eISSN - 1442-1984
pISSN - 0913-557X
DOI - 10.1111/1442-1984.12156
Subject(s) - biology , genetic diversity , genetics , loss of heterozygosity , primer (cosmetics) , evolutionary biology , population , genetic structure , genetic variation , allele , gene , chemistry , demography , organic chemistry , sociology
EST‐SSR markers were developed for A sarum sakawanum var. stellatum to evaluate the genetic diversity and genetic structure of series S akawanum and assess their utility in other series in the genus Asarum . A total of 1864 primer pairs, including 1864 tri‐nucleotide SSR motifs, were designed based on transcriptome data. Of the 96 primers tested, 17 loci were successfully amplified and polymorphic in series S akawanum taxa. Furthermore, all these loci were successfully amplified in A. takaoi , whereas only 10 loci were amplified in the more phylogenetically distant A . pellucidum . For populations of A . sakawanum var. stellatum , the values of observed and expected heterozygosity ranged from 0.261 to 0.957 and 0.317 to 0.881, respectively. These newly developed EST‐SSR markers will be used in population genetic studies of series S akawanum to infer the evolutionary history and genetic diversity of these series, many of which are endangered in J apan.