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Isolation and characterization of L igustrum micranthum ( O leaceae) microsatellite loci using paired‐end I llumina reads
Author(s) -
Sugai Kyoko,
Setsuko Suzuki
Publication year - 2017
Publication title -
plant species biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 36
eISSN - 1442-1984
pISSN - 0913-557X
DOI - 10.1111/1442-1984.12119
Subject(s) - microsatellite , biology , linkage disequilibrium , locus (genetics) , genetics , population , allele , primer (cosmetics) , gene , haplotype , chemistry , demography , sociology , organic chemistry
Twenty‐six microsatellite loci were developed and characterized for L igustrum micranthum , a species endemic to the O gasawara I slands, J apan. The genetic structure of this species must be clarified in order to restore the island's ecosystem. A total of 8511 primer pairs were designed from de novo sequencing. Of the 48 primer pairs selected, amplification and polymorphisms were tested using one population each from the C hichijima and Hahajima Islands of the O gasawara I slands. Twenty‐six microsatellite loci were successfully amplified and the number of alleles for these loci ranged from five to 31 per locus, and the mean expected heterozygosities were 0.858 and 0.849, respectively. No significant deviation from the H ardy– W einberg equilibrium was observed in either population, and no significant linkage disequilibrium was detected between any locus pair. The microsatellite loci reported in this study can be used in future studies to evaluate the genetic structure and mating system of L . micranthum .