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Development and characterization of polymorphic microsatellite markers for N eolitsea sericea using I llumina paired‐end draft sequencing data
Author(s) -
Moriguchi Yoshinari,
Yamanaka Haruna,
Ohdan Takashi,
Fuji Shinichi
Publication year - 2016
Publication title -
plant species biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 36
eISSN - 1442-1984
pISSN - 0913-557X
DOI - 10.1111/1442-1984.12097
Subject(s) - biology , microsatellite , genetics , population , polymorphism (computer science) , primer (cosmetics) , allele , gene , demography , sociology , chemistry , organic chemistry
Simple sequence repeat ( SSR ) markers were developed and characterized for N eolitsea sericea ( B l.) K oidz. ( L auraceae). Out of 196 designed primer pairs, a total of 144 pairs showed amplification, of which 44 had clear and stable chromatograms. Polymorphism of these 44 loci was tested using 32 individuals sampled from a single population of N . sericea . The number of alleles and the polymorphism information content varied from 3 to 12 and 0.271 to 0.853, respectively. A significant departure from the H ardy‐ W einberg equilibrium was observed in one of the 44 loci. These SSR markers are useful for population genetic studies and parentage analysis in N . sericea , which is one of the most common evergreen species in coastal P inus thunbergii forests in central‐western J apan.