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Isolation and characterization of novel microsatellite markers for C ymodocea serrulata ( C ymodoceaceae), a seagrass distributed widely in the I ndo‐ P acific region
Author(s) -
Arriesgado Dan M.,
Kurokochi Hiroyuki,
Lian Chunlan,
Nakajima Yuichi,
Matsuki Yu,
Nagai Satoshi,
Yasuike Motoshige,
Nakamura Yoji,
Uy Wilfredo H.,
Fortes Miguel D.,
Campos Wilfredo L.,
Nadaoka Kazuo
Publication year - 2015
Publication title -
plant species biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 36
eISSN - 1442-1984
pISSN - 0913-557X
DOI - 10.1111/1442-1984.12064
Subject(s) - biology , microsatellite , genotyping , genetic diversity , allele , locus (genetics) , genetics , genetic structure , population , genotype , genetic variation , gene , demography , sociology
C ymodocea serrulata is a tropical seagrass species distributed widely in the I ndo‐ P acific region. We developed 16 novel microsatellite (simple sequence repeat) markers for C . serrulata using next‐generation sequencing for use in genetic studies. The applicability of these markers was attested by genotyping of 40 individuals collected from a natural population in the P hilippines. Of the 16 loci, 15 showed polymorphism. For the 15 polymorphic markers, the number of alleles per locus ranged from two to seven, and the observed and expected heterozygosities ranged from 0.131–1.000 and 0.124–0.788, respectively. These markers are useful tools for elucidating genetic diversity, connectivity, and structure in this foundational coastal species.