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Development of microsatellite markers for Aquilegia buergeriana var. oxysepala ( R anunculaceae), a vulnerable J apanese herb
Author(s) -
Itagaki Tomoyuki,
Kimura Megumi K.,
Lian Chunlan,
Sakai Satoki
Publication year - 2015
Publication title -
plant species biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 36
eISSN - 1442-1984
pISSN - 0913-557X
DOI - 10.1111/1442-1984.12044
Subject(s) - biology , microsatellite , locus (genetics) , genetic diversity , herb , allele , population , genetics , genotyping , botany , genotype , gene , traditional medicine , medicinal herbs , demography , sociology , medicine
Aquilegia buergeriana var. oxysepala is a vulnerable herb, for which 20 microsatellite markers were developed. The applicability of these markers was confirmed by genotyping of 66 individuals collected from four natural populations in J apan. Of the 20 loci, 15 showed polymorphism. For the 15 polymorphic markers, the number of alleles per locus ranged from 2 to 12, and the expected and observed heterozygosities ranged from 0.13 to 0.77 and 0.02 to 0.65, respectively. The markers described here are sufficiently polymorphic and informative to investigate the genetic diversity and population genetic structure of A. buergeriana var. oxysepala .