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Dysfunction of perivascular adipose tissue in mesenteric artery is restored by aerobic exercise in high‐fat diet induced obesity
Author(s) -
Liao Jingwen,
Yin Honggang,
Huang Junhao,
Hu Min
Publication year - 2021
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.13404
Subject(s) - endocrinology , medicine , adipose tissue , mesenteric arteries , aerobic exercise , artery , chemistry
This study investigated the function of perivascular adipose tissue (PVAT) on vascular contractility within resistant arteries in high‐fat diet induced obese rats after long‐term aerobic exercise. Male Sprague‐Dawley rats were subjected to normal diet control group (N‐CTRL), normal diet exercise group (N‐EX), high‐fat diet control group (H‐CTRL), and high‐fat diet exercise group (H‐EX) (n = 8 in each group). After intervention, adipose tissues morphology was observed. Vasomotor function of mesenteric arteries with or without PVAT were assessed; mesenteric PVAT isolated from each group were transferred to chambers bath with untreated vessels (without PVAT) to evaluate the independent effect. Isolated PVAT was further pre‐treated with inhibitor of cystathionine‐γ‐lyase (CSE), a key hydrogen sulphide (H 2 S) enzyme. Results showed that the size of lipid droplet around mesenteric arteries from H‐EX was significantly reduced ( P < .05); uncoupling protein1 (UCP1) in PVAT from H‐EX was enhanced. In N‐CTRL, N‐EX, and H‐EX, vessels without PVAT showed higher sensitivity to serotonin (5‐HT) than that with intact PVAT. Vascular tension by 5‐HT was significantly reduced in H‐EX than H‐CTRL ( P < .05) in vessels with PVAT. Transferred PVAT from H‐EX compared with H‐CTRL significantly reduced vascular sensitivity to 5‐HT ( P < .05), and this effect was eliminated through inhibiting CSE. In summary, the anti‐contractile effect of PVAT on resistance artery was impaired in obesity but restored by long‐term aerobic exercise. The function of PVAT modified by obesity or by exercise has an independent influence on vascular reactivity, and PVAT derived H 2 S may participate in this process.