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Effects of ticagrelor pretreatment on electrophysiological properties of stellate ganglion neurons following myocardial infarction
Author(s) -
Cheng Lijun,
Fu Huaying,
Wang Xinghua,
Ye Lan,
Lakhani Ishan,
Tse Gary,
Zhang Zhiwei,
Liu Tong,
Li Guangping
Publication year - 2020
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.13385
Subject(s) - electrophysiology , medicine , patch clamp , chemistry , potassium channel , endocrinology , stellate ganglion , context (archaeology) , potassium , cardiology , pharmacology , biology , pathology , paleontology , alternative medicine , organic chemistry
Higher sympathetic activity predisposes to malignant ventricular arrhythmias in the context of myocardial infarction (MI). This is, in part, mediated by the electrical activity of the stellate ganglion (SG). The aim of this study is to examine the effects of ticagrelor pretreatment on the electrophysiological properties of SG neurons following MI in rabbits. MI was induced by isoproterenol (ISO) of 150 mg kg ‐1 d ‐1 (twice at an interval of 24 hours). Ticagrelor pretreatment was administered at low‐ (10 mg kg ‐1 d ‐1 ) or high‐dose (20 mg kg ‐1 d ‐1 ). Protein and RNA expression were determined by immunohistochemical analysis and real‐time PCR, respectively. The activity of sodium channel current ( I Na ), delayed rectifier potassium current ( I KDR ), M‐type potassium current ( I KM ) as well as action potentials (APs) from SG neurons were measured by whole‐cell patch‐clamp. Intracellular calcium concentrations were measured by confocal microscopy. Compared with the control group, the MI group exhibited a greater amplitude of I Na , I KDR and I KM , significantly altered activation and inactivation characteristics of I Na , no significant alterations in protein or mRNA expression of sodium and M‐type potassium channels, along with higher AP amplitude and frequency and intracellular calcium concentrations. Most of these abnormalities were prevented by pretreatment with low‐ or high‐dose ticagrelor. Our data suggest that ticagrelor exerts cardioprotective effects, potentially through modulating the activity of different ion channels in SG neurons.