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Iron alters Ca 2+ homeostasis in doxorubicin‐resistant K562 cells
Author(s) -
Yalcintepe Leman,
Erdag Demet,
Akbas Fahri,
Kucukkaya Bahire
Publication year - 2020
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.13295
Subject(s) - homeostasis , calcium metabolism , calcium , k562 cells , metabolism , intracellular , biology , gene expression , microbiology and biotechnology , doxorubicin , cell growth , calcium in biology , cell culture , chemistry , cell , gene , endocrinology , biochemistry , genetics , organic chemistry , chemotherapy
Iron is an essential trace element especially in cell proliferation, and growth for various cellular events. An increasing amount of research has shown that iron metabolism is altered in tumour cells which usually have rapid growth rates. However, the number of studies on iron metabolism, and calcium regulation are limited in drug‐resistant tumour cells. Previously, we have shown that modulation of iron metabolism through iron chelation regulated the intracellular calcium, and increased the doxorubicin sensitivity. In the present study, we investigated the effects of iron on mRNA expression profiles of fifteen key genes (IP 3 R1/2/3, RYR1/2, SERCA1/2/3, NCX1/2/3, PMCA1/2/3, and PMCA4) related to calcium homeostasis in the parental cell line K562 and its subclone doxorubicin‐resistant K562 cells. According to the ΔΔCt method with a two‐fold expression difference ( P < .05) as a cut‐off level, although iron showed differential effects on most of the genes, IP 3 R and PMCA genes were especially determined to have changed significantly. These results show that iron metabolism is an important metabolism due to changes in the expression of genes involved in calcium regulation and is a new perspective to overcome cancer/drug resistance.