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Alpha‐pinene promotes osteoblast differentiation and attenuates TNFα‐induced inhibition of differentiation in MC3T3‐E1 pre‐osteoblasts
Author(s) -
Min HyeonYoung,
Son HyoEun,
Jang WonGu
Publication year - 2020
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.13245
Subject(s) - osteoblast , chemistry , alkaline phosphatase , mineralization (soil science) , extracellular matrix , tumor necrosis factor alpha , microbiology and biotechnology , biochemistry , endocrinology , enzyme , biology , organic chemistry , in vitro , nitrogen
Alpha‐pinene (α‐pinene) is an organic compound, found in the oils of many species of coniferous trees, especially pine. α‐Pinene reportedly has antioxidant and anti‐inflammatory activities; however, its effects on osteoblasts are unknown. This study investigated the effects of α‐pinene on osteoblast differentiation and tumour necrosis factor‐alpha (TNFα)‐induced inhibition of osteogenesis. Culture in control or osteogenic medium containing α‐pinene increased osteogenic marker expression. Alkaline phosphatase staining and alizarin red S staining confirmed that α‐pinene enhanced osteoblast differentiation. Also, α‐pinene attenuated TNFα‐induced inhibition of Smad1/5/9 phosphorylation and extracellular matrix mineralization. Taken together, our findings suggest that α‐pinene enhances osteoblast differentiation and mineralization in MC3T3‐E1 pre‐osteoblasts.