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MicroRNA‐147b suppresses the proliferation and invasion of non‐small‐cell lung cancer cells through downregulation of Wnt/β‐catenin signalling via targeting of RPS15A
Author(s) -
Ning Qian,
Pang Yamei,
Shao Shan,
Luo Minna,
Zhao Lin,
Hu Tinghua,
Zhao Xinhan
Publication year - 2020
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.13203
Subject(s) - wnt signaling pathway , microrna , cancer research , downregulation and upregulation , oncogene , biology , cell growth , lung cancer , cancer , catenin , signal transduction , microbiology and biotechnology , medicine , cell cycle , gene , genetics
Deregulation of microRNAs (miRNAs) leads to malignant growth and aggressive invasion during cancer occurrence and progression. miR‐147b has emerged as one of the cancer‐related miRNAs that are dysregulated in multiple cancers. Yet, the relevance of miR‐147b in non‐small‐cell lung cancer (NSCLC) remains unclear. In the present study, we aimed to report the biological function and signalling pathways mediated by miR‐147b in NSCLC. Our results demonstrate that miR‐147b expression is significantly downregulated in NSCLC tissues and cell lines. Overexpression of miR‐147b decreased the proliferative ability, colony‐forming capability, and invasive potential of NSCLC cells. Notably, our study identified ribosomal protein S15A (RPS15A), an oncogene in NSCLC, as a target gene of miR‐147b. Our results showed that miR‐147b negatively modulates RPS15A expression in NSCLC cells. An inverse correlation between miR‐147b and RPS15A was evidenced in NSCLC specimens. Moreover, miR‐147b overexpression downregulated the activation of Wnt/β‐catenin signalling via targeting of RPS15A. Overexpression of RPS15A partially reversed the miR‐147b‐mediated antitumour effect in NSCLC cells. Collectively, these findings reveal that miR‐147b restricts the proliferation and invasion of NSCLC cells by inhibiting RPS15A‐induced Wnt/β‐catenin signalling and suggest that the miR‐147b/RPS15A/Wnt/β‐catenin axis is an important regulatory mechanism for malignant progression of NSCLC.