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Effects of ANP on pulmonary vein electrophysiology, Ca 2+ homeostasis and adrenergic arrhythmogenesis via PKA
Author(s) -
Lu YenYu,
Cheng ChenChuan,
Wu HuiJun,
Lin YungKuo,
Chen YaoChang,
Chen ShihAnn,
Chen YiJen
Publication year - 2020
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.13199
Subject(s) - medicine , endocrinology , chemistry , homeostasis , electrophysiology , atrial natriuretic peptide , agonist , patch clamp , endoplasmic reticulum , calcium , pulmonary vein , atrial fibrillation , receptor , biochemistry
Atrial fibrillation (AF) is the most common form of arrhythmia and increases the risk of stroke and heart failure (HF). Pulmonary veins (PVs) are important sources of triggers that generate AF, and calcium (Ca 2+ ) overload participates in PV arrhythmogenesis. Neurohormonal activation is an important cause of AF. Higher atrial natriuretic peptide (ANP) level predicts paroxysmal AF occurrence in HF patients. However, it is not clear if ANP directly modulates electrophysiological characteristics and Ca 2+ homeostasis in the PVs. Conventional microelectrodes, whole‐cell patch‐clamp, and the Fluo‐3 fluorimetric ratio technique were performed using isolated rabbit PV preparations or single isolated PV cardiomyocytes before and after ANP administration. We found that ANP (1, 10, and 100 nmol/L) concentration‐dependently decreased spontaneous activity in PV preparations. ANP (100 nmol/L) decreased isoproterenol (1 μmol/L)‐induced PV spontaneous activity and burst firing. AP811 (100 nmol/L, NPR‐C agonist), H89 (1μmol/L, PKA inhibitor) decreased isoproterenol‐induced PV spontaneous activity or burst firing, but successive administration of ANP had no further effect on PV activity. KT5823 (1 μmol/L, PKG inhibitor) decreased isoproterenol‐induced PV spontaneous activity but did not change isoproterenol‐induced PV burst firing, whereas successive administration of ANP did not change isoproterenol‐induced PV burst firing. ANP decreased intracellular Ca 2+ transient and sarcoplasmic reticulum Ca 2+ content in single PV cardiomyocytes. ANP decreased the late sodium current, L‐type Ca 2+ current, but did not change nickel‐sensitive Na + ‐Ca 2+ exchanger current in single PV cardiomyocytes. In conclusion, ANP directly regulates PV electrophysiological characteristics and Ca 2+ homeostasis and attenuates isoproterenol‐induced arrhythmogenesis through NPR‐C/cAMP/PKA signal pathway.