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Effects of nitric oxide on apoptosis and voltage‐gated calcium channels in human cardiac myofibroblasts
Author(s) -
Bae Hyemi,
Kim Taeho,
Lim Inja
Publication year - 2020
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.13178
Subject(s) - snap , microbiology and biotechnology , apoptosis , chemistry , nitric oxide , patch clamp , myofibroblast , channel blocker , nitric oxide synthase , calcium , biology , medicine , biochemistry , receptor , computer graphics (images) , organic chemistry , computer science , fibrosis
Summary We characterised the voltage‐gated Ca 2+ channels ( VGCC s) in human cardiac fibroblasts ( HCF s) and myofibroblasts ( HCMF s) and investigated the effects of nitric oxide ( NO ) on apoptosis and on these channels. Western blotting and immunofluorescence analyses show that α‐smooth muscle actin (a myofibroblast marker) was markedly expressed in passage (P) 12‐15 but not in P4 HCF cells, whereas calponin (a fibroblast marker) was expressed only in P4 cells. Ca V 1.2 (L‐type) and Ca V 3.3 (T‐type) of VGCC s were highly expressed in P12‐15 cells, but only weak Ca V 2.3 (R‐type) expression was identified in P4 cells using reverse transcription‐polymerase chain reaction analysis. S‐Nitroso‐N‐acetylpenicillamine ( SNAP , an NO donor) decreased cell viability of HCMF s in a dose‐dependent manner and induced apoptotic changes, and nifedipine (an L‐type Ca 2+ channel blocker) prevented apoptosis as shown with immunofluorescence staining and flow cytometry. Whole‐cell mode patch‐clamp recordings demonstrate the presence of L‐type Ca 2+ ( I C a,L ) and T‐type Ca 2+ ( I C a,T ) currents in HCMF s. SNAP inhibited I C a,L of HCMF s, but pre‐treatment with ODQ (a guanylate cyclase inhibitor) or KT 5823 (a PKG inhibitor) prevented it. Pre‐treating cells with KT 5720 (a PKA inhibitor) or SQ 22536 (an adenylate cyclase inhibitor) blocked SNAP ‐induced inhibition of I C a,L . 8‐Bromo‐cyclic GMP or 8‐bromo‐cyclic AMP also inhibited I C a,L . However, pre‐treatment with N‐ethylmaleimide (a thiol‐alkylating reagent) did not block the SNAP effect, nor did DL ‐dithiothreitol (a reducing agent) reverse it. These data suggest that high concentrations of NO injure HCMF s and inhibit I C a,L through the PKG and PKA signalling pathways but not through the S ‐nitrosylation pathway.