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Micro RNA ‐198‐5p inhibits the migration and invasion of non‐small lung cancer cells by targeting fucosyltransferase 8
Author(s) -
Wang Siyao,
Zhang Xin,
Yang Chunlu,
Xu Shun
Publication year - 2019
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.13154
Subject(s) - cancer research , metastasis , western blot , lung cancer , downregulation and upregulation , cell migration , epithelial–mesenchymal transition , fucosyltransferase , transfection , microrna , blot , biology , cancer , cell , medicine , pathology , cell culture , gene , biochemistry , genetics
Micro RNA ‐198‐5p (miR‐198‐5p) displays crucial roles in various cancers including non‐small cell lung cancer ( NSCLC ), but the underlying molecular mechanisms remain unclear. Fucosyltransferase 8 ( FUT 8) is associated with tumour metastasis and prognosis. In this study, we explored the expression of miR‐198‐5p and FUT 8 in NSCLC patients. Results showed that miR‐198‐5p was under‐expressed in NSCLC tissues and was negatively correlated with tumour size, lymph node metastasis and tumour‐node‐metastasis stage, while FUT 8 expression was highly upregulated. Next, we altered miR‐198‐5p expression using the mimic or inhibitor in the functional study. Results showed that miR‐198‐5p overexpression could inhibit the migration, invasion and epithelial‐to‐mesenchymal transition ( EMT ) of NSCLC cells; reversely, suppression of miR‐198‐5p enhanced cell migration, invasion and EMT . In vivo, miR‐198‐5p overexpression inhibited the formation of mouse lung and liver metastasis. Luciferase reporter, real‐time PCR and western blot assays showed that miR‐198‐5p could directly target FUT 8 and regulate FUT 8 expression. Further, FUT 8 overexpression reversed the effect of miR‐198‐5p overexpression on the migration, invasion and EMT of NSCLC cells. Taken together, miR‐198‐5p functions as a tumour suppressor by targeting FUT 8 in NSCLC . MiR‐198‐5p may be developed as a new diagnostic biomarker and therapeutic target for lung cancer.