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Hypoxic pulmonary vasoconstriction is lacking in rats with type 1 diabetes
Author(s) -
Soloviev Anatoly,
Ivanova Irina,
Melnyk Mariia,
Dobrelia Nataliia,
Khromov Alexander
Publication year - 2019
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.13137
Subject(s) - hypoxic pulmonary vasoconstriction , vasoconstriction , cardiology , medicine
Hypoxic pulmonary vasoconstriction (HPV) is the most important feature of intact lung circulation that matches local blood perfusion to ventilation. The main goal of this work was to study the effects of diabetes on the development of HPV in rats. The experimental design comprised diabetes mellitus induction by streptozotocin, video‐morphometric measurements of the lumen area of intrapulmonary arteries (iPAs) using perfused lung tissue slices and patch‐clamp techniques. It was shown that iPA lumen size was significantly reduced under physical and chemical hypoxia (7–10 mm Hg) in normal iPA, but, on the contrary, it clearly increased in diabetic lung slices. The amplitude of the outward K + current in diabetic iPAs smooth muscle cells (SMCs) was two‐fold greater than that seen in healthy cells. Chemical hypoxia led to significant decrease in the amplitude of the K + outward current in healthy iPA SMCs while it was without effect in diabetic cells. The data obtained clearly indicate a significant dysregulation of vascular tone in pulmonary circulation under diabetes, ie diabetes damages the adaptive mechanism for regulating blood flow from poorly ventilated to better ventilated regions of the lung under hypoxia. This effect could be clinically important for patients with diabetes who have acute or chronic lung diseases associated with the lack of blood oxygenation.