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Esculetin inhibits histamine‐induced expression of inflammatory cytokines and mucin in nasal epithelial cells
Author(s) -
Sun Bin,
Wang Botao,
Xu Min
Publication year - 2019
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.13128
Subject(s) - histamine , mucin , chemistry , western blot , pharmacology , coumarin , inflammation , proinflammatory cytokine , nf κb , secretion , in vitro , immunology , biochemistry , medicine , signal transduction , gene , organic chemistry
Allergic rhinitis ( AR ) is a type of respiratory disease closely associated with chronic inflammation. Esculetin is a natural coumarin derivative and has been reported to possess anti‐allergic and anti‐inflammatory effects. However, the roles of esculetin in AR have not been studied. In this study, we aimed to examine the effect of esculetin on AR using an in vitro model. The human nasal epithelial cells ( HNE pC) were stimulated by histamine for 24 hours with or without the pretreatment of esculetin. The mRNA levels and production of inflammatory cytokines including IL ‐6 and IL ‐8, as well as mucin 5 AC ( MUC 5 AC ) were measured using qRT ‐ PCR and ELISA , respectively. The results showed that esculetin suppressed histamine‐induced expression and secretion of IL ‐6, IL ‐8, and MUC 5 AC in HNE pCs. Furthermore, we examined the effect of esculetin on NF ‐κB pathway by detecting the expression levels of NF ‐κB p65, p‐p65 and IκBα using western blot analysis. Esculetin treatment suppressed the histamine‐induced p‐p65 expression and p‐IκBα degradation. Inhibiting NF ‐κB pathway suppressed histamine‐induced production of IL ‐6, IL ‐8, and MUC 5 AC in HNE pCs. These findings suggested that esculetin suppressed histamine‐induced production of inflammatory cytokines and mucin in HNE pCs, which were partly mediated by the inhibition of NF ‐κB pathway.