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KCNQ 1 OT 1, HIF 1A‐ AS 2 and APOA 1‐ AS are promising novel biomarkers for diagnosis of coronary artery disease
Author(s) -
Zhang Yuan,
Zhang Lei,
Wang Yu,
Ding Han,
Xue Sheng,
Yu Hua,
Hu Longgang,
Qi Hongzhao,
Wang Yin,
Zhu Wenjie,
Liu Dacheng,
Li Peifeng
Publication year - 2019
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.13094
Subject(s) - hif1a , peripheral blood mononuclear cell , coronary artery disease , real time polymerase chain reaction , biomarker , long non coding rna , medicine , biology , rna , microbiology and biotechnology , cancer research , chemistry , angiogenesis , gene , biochemistry , in vitro
This study aimed to evaluate the potential of long noncoding RNA s (lnc RNA s) as biomarkers for coronary artery disease ( CAD ). We measured the levels of three atherosclerosis‐ or cardiac‐related lnc RNA s in peripheral blood monocyte cells ( PBMC s) from 20 CAD patients and 20 non‐ CAD control participants using real‐time reverse transcription–polymerase chain reaction (real‐time RT – PCR ) methods. We found that the levels of lnc RNA KCNQ 1 opposite strand/antisense transcript 1 ( KCNQ 1 OT 1), hypoxia‐inducible factor 1 alpha‐antisense RNA 2 ( HIF 1A‐ AS 2) and apolipoprotein A‐1 antisense RNA ( APOA 1‐ AS ) were significantly increased in CAD patients ( KCNQ 1 OT 1 increased by 2.38‐fold, P = 0.00042; HIF 1A‐ AS 2 increased by 2.00‐fold, P = 0.0001; APOA 1‐ AS increased by 4.52‐fold, P = 0.000048). The area under the ROC curve was 0.865 for KCNQ 1 OT 1, 0.852 for HIF 1A‐ AS 2, and 0.967 for APOA 1‐ AS . Furthermore, the combination of lnc RNA s resulted in a much higher AUC value of 0.990 for the prediction of CAD . Spearman's correlation analysis showed that APOA 1‐ AS was positively correlated with NT ‐pro BNP , CKMB , MYO and HsTnT, whereas HIF 1A‐ AS 2 was correlated with NT ‐pro BNP and HsTnT. Hence, the elevation of KCNQ 1 OT 1, HIF 1A‐ AS 2 and APOA 1‐ AS predicts CAD and these molecules may be considered as novel biomarkers of CAD .