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The Gn RH analogues affect novel neuropeptide SMIM 20/phoenixin and GPR 173 receptor expressions in the female rat hypothalamic–pituitary–gonadal ( HPG ) axis
Author(s) -
SuszkaŚwitek Aleksandra,
Pałasz Artur,
Filipczyk Łukasz,
Menezes Itiana Castro,
MordeckaChamera Kinga,
Angelone Tommaso,
Bogus Katarzyna,
Bacopoulou Flora,
Worthington John J.,
Wiaderkiewicz Ryszard
Publication year - 2019
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.13061
Subject(s) - buserelin , hypothalamic–pituitary–gonadal axis , medicine , endocrinology , agonist , hypothalamus , neuropeptide , hormone , gonadotropin releasing hormone , receptor , antagonist , messenger rna , biology , chemistry , luteinizing hormone , biochemistry , gene
Summary The recently discovered peptide phoenixin ( PNX ) and its receptor GPR 173 are novel factors that exhibit a large spectrum of regulatory activity, especially when considered as a central modulator of Gn RH ‐related hormonal control of reproductive processes. It has been already proven that Gn RH agonists and antagonists can modulate peptidergic signalling in the HPG axis. Despite these findings, there is so far no information regarding the influence of treatment with Gn RH analogues on SMIM 20/phoenixin signalling in the hypothalamic–pituitary–gonadal axis. In the current study, SMIM 20/phoenixin and GPR 173 mRNA levels were measured in the hypothalamus, pituitary and ovaries of female rats in the dioestrus phase following treatment with Gn RH ‐R agonist (buserelin) and antagonist (cetrorelix) using quantitative real‐time PCR . The serum PNX concentrations were also estimated with ELISA technique. The hypothalamic, hypophyseal and especially ovarian levels of SMIM 20 mRNA were increased after both buserelin and cetrorelix administration. The GPR 173 expressions were in turn decreased in the hypothalamus and pituitary. Treatment with the Gn RH analogues led to the modulation of SMIM 20/phoenixin and GPR 173 mRNA expression in the female rat hypothalamic–pituitary–gonadal axis. By identifying buserelin and cetrorelix as novel modulators of phoenixin signalling in the animal HPG axis, these results cast new light on the Gn RH analogues mode of action and contribute to a better understanding of the mechanisms responsible for the hormonal control of reproduction.