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Evaluation of mitochondrial respiratory function in rat cardiomyocytes under different glucose conditions, using an extracellular flux analysis method
Author(s) -
Tsubone Hirokazu,
Mutoh Tatsushi
Publication year - 2018
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.13014
Subject(s) - extracellular , anaerobic exercise , mitochondrion , respiration , respiratory system , oxygen , flux (metallurgy) , extracellular fluid , carbohydrate metabolism , atp synthase , biochemistry , citrate synthase , chemistry , cellular respiration , medicine , biophysics , biology , anatomy , enzyme , physiology , organic chemistry
Summary We studied the effect of glucose supply on mitochondrial respiratory function ( MRF ) of neonatal rat cardiomyocytes, using a novel extracellular flux analysis. Fundamental respiratory parameters regarding oxygen consumption rate in mitochondria showed glucose concentration‐dependent changes, where a significant increase or decrease in these parameters was observed to be associated with glucose concentrations ranging between 10% and 1000% of the concentration used in standard medium (3151 mg/L), respectively. By contrast, the extracellular acidification rate, a parameter of anaerobic activity, was shown to decrease under low‐glucose conditions, whereas it increased after inhibiting complex V ( ATP synthase) under the same glucose conditions. These findings provide a beneficial basis for various experimental studies on mitochondrial metabolism in cardiomyocytes.