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Vitamin B complex mitigates cardiac dysfunction in high‐methionine diet‐induced hyperhomocysteinemia
Author(s) -
Jeremic Jovana,
Nikolic Turnic Tamara,
Zivkovic Vladimir,
Jeremic Nevena,
Milosavljevic Isidora,
Srejovic Ivan,
Obrenovic Radmila,
Jancic Snezana,
Rakocevic Milena,
Matic Stevan,
Djuric Dragan,
Jakovljevic Vladimir
Publication year - 2018
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.12930
Subject(s) - hyperhomocysteinemia , methionine , medicine , homocysteine , endocrinology , cardiac function curve , vitamin , b vitamins , vitamin b12 , heart failure , biology , amino acid , biochemistry
Summary This research is designed to test the hypothesis that elevated homocysteine (Hcy) levels in vivo, caused by a deficit in vitamin B complex, promote changes in cardiac function and redox status that lead to heart failure. In order to conduct the study, we used adult male Wistar albino rats (n = 30; 4 weeks old; 100 ± 15 g body weight). Hyperhomocysteinaemia ( HH cy) in these animals was achieved by dietary manipulation. For 4 weeks, the animals were fed with a standard rodent chow (control, CF ), a diet enriched in methionine with no deficiency in B vitamins (i.e., folic acid, B6 and B12) ( HMNV ) or a diet enriched in methionine and deficient in B vitamins ( HMLV ). After 28 days of dietary manipulation, all animals were killed. The rat hearts were isolated and retrogradely perfused according to the Langendorff technique at a gradually increasing perfusion pressure. We found a negative correlation between elevated serum Hcy and total body and heart weight. The maximum rate of left ventricular pressure development was significantly increased in the HMNV group compared with in the other groups. Systolic left ventricular pressure was significantly changed in all groups. HH cy induces remodelling of the cardiac tissues, as moderate HH cy is associated with more prominent interstitial and perivascular fibrosis. Our results suggest that a high methionine diet without vitamin B complex causes profound negative effects associated with HH cy.