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Quantitative proteomics reveals decreased expression of major urinary proteins in the liver of apoE/ eNOS ‐ DKO mice
Author(s) -
Stachowicz Aneta,
Olszanecki Rafał,
Suski Maciej,
Wiśniewska Anna,
Kuś Katarzyna,
Białas Magdalena,
Jawień Jacek,
Korbut Ryszard
Publication year - 2018
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.12927
Subject(s) - enos , endocrinology , medicine , apolipoprotein e , lipid metabolism , biology , mitochondrial biogenesis , biochemistry , chemistry , nitric oxide synthase , nitric oxide , mitochondrion , disease
Summary Endothelial nitric oxide synthase ( eNOS )‐derived nitric oxide ( NO ) plays an important role, not only in endothelium‐dependent vasodilation but also in lipid and glucose homeostasis in the liver and exerts beneficial effects on mitochondrial biogenesis and respiration. Thus, the aim of our study was to use iTRAQ ‐based quantitative proteomics to investigate the changes in protein expression in the mitochondrial and cytosolic fractions isolated from the liver of the double (apolipoprotein E (apoE) and eNOS ) knockout (apoE/ eNOS ‐ DKO ) mice as compared to apoE KO mice (apoE −/− ) – an animal model of atherosclerosis and hepatic steatosis. Collectively, the deficiency of eNOS resulted in increased expression of proteins related to gluconeogenesis, fatty acids and cholesterol biosynthesis as well as the decreased expression of proteins participated in triglyceride breakdown, cholesterol transport, protein transcription & translation and processing in endoplasmic reticulum ( ER ). Moreover, one of the most downregulated proteins were major urinary proteins ( MUP s), which are abundantly expressed in the liver and were shown to be involved in the regulation of lipid and glucose metabolism. The exact functional consequences of the revealed alterations require further investigation.