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rs294775 is a cis ‐regulatory SNP for human UGT 2B10
Author(s) -
Ruan Ji,
Yang YuChen,
Long FuQuan,
Sun Chang
Publication year - 2018
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.12925
Subject(s) - mutagenesis , snp , chemistry , enzyme , luciferase , biochemistry , single nucleotide polymorphism , pharmacology , mutation , biology , gene , genotype , transfection
Summary UGT 2B10 is an important metabolism enzyme in human body and its substrates include multiple amine‐containing compounds, especially nicotine, tamoxifen and multiple antidepressants. Multiple common SNP s have been observed in its promoter region, but their role in expression regulation has never been investigated. In this preliminary study, we identified a novel cis ‐regulatory SNP , rs294775, for UGT 2B10 by plasmid construction, mutagenesis, and luciferase assay, whose mechanism was also investigated. Our work provides a basis for further pharmacogenetics study.