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Ptrf transgenic mice exhibit obesity and fatty liver
Author(s) -
Li Qian,
Bai Lin,
Shi Guiying,
Zhang Lianfeng,
Dai Yifan,
Liu Pingsheng,
Cong YuSheng,
Wang Miao
Publication year - 2018
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.12920
Subject(s) - transgene , genetically modified mouse , fatty liver , biology , knockout mouse , in vivo , microbiology and biotechnology , chemistry , medicine , gene , biochemistry , genetics , disease
Summary Polymerase I and transcript release factor ( Ptrf , also known as Cavin1 ) is an essential component in the biogenesis and function of caveolae. Ptrf knockout mice or patients with PTRF mutations exhibit numerous pathologies including markedly aberrant fuel metabolism, lipodystrophy and muscular dystrophy. In this study, we generated Ptrf transgenic mice to explore its function in vivo . Compared with wild‐type ( WT ) mice, we found that the Ptrf transgenic mice showed obesity with an increased level of ALT (alanine aminotransferase) and AST (aspartate transaminase). Ptrf transgenic mice exhibited severe fat degeneration and a higher degree of fat accumulation in the liver compared with WT mice. Consistently, we found that the expression of the fat synthesis gene, Fasn , was increased in the liver of Ptrf transgenic mice. Thus, Ptrf transgenic mice would be a good model for investigating the molecular mechanism and therapeutic targets of obesity and fatty liver associated diseases.