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MiR‐96 regulates bone metabolism by targeting osterix
Author(s) -
Liu Hua,
Liu Qing,
Wu XianPing,
He HongBo,
Fu Lei
Publication year - 2018
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.12912
Subject(s) - microrna , mesenchymal stem cell , bone remodeling , osteoporosis , bone marrow , endocrinology , medicine , chemistry , microarray analysis techniques , microbiology and biotechnology , gene expression , biology , gene , biochemistry
Summary Micro RNA s (mi RNA s) play important roles in bone metabolism and aging. Here we show that miR‐96 was markedly up‐regulated in serum of elderly patients with osteoporosis by mi RNA microarray analysis and qRT ‐ PCR . Moreover miR‐96 was also up‐regulated in bone marrow mesenchymal stem cells ( BMSC s) of aged humans and mice. Our results show that the over‐expression of miR‐96 reduced osteogenic differentiation of BMSC s, whereas the inhibition of miR‐96 increased osteogenic differentiation of BMSC s. At the molecular level, miR‐96 regulated osteogenesis by targeting osterix. Interestingly, over‐expression of miR‐96 in young mice by intravenous injection of agomiR‐96 developed a low bone mass due to impaired osteogenesis. However, inhibition of miR‐96 in aged mice attenuated the age‐related bone loss. Thus, our data suggest that miR‐96 regulates osteogenesis and may represent a potential diagnostic marker or therapeutic target for age‐related bone loss.