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Dopamine D1 receptors induce apoptosis of osteosarcoma cells via changes of MAPK pathway
Author(s) -
Gao Jun,
Gao Feng
Publication year - 2017
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.12814
Subject(s) - mapk/erk pathway , p38 mitogen activated protein kinases , apoptosis , microbiology and biotechnology , kinase , agonist , chemistry , phosphorylation , protein kinase a , mitogen activated protein kinase 3 , signal transduction , receptor , cancer research , biology , biochemistry
Summary This study explored the effects and mechanisms of dopamine D1 receptors ( DR 1) activation on the apoptosis of osteosarcoma cells ( OS 732).The DR 1 agonist SKF ‐38393 decreased the viability of OS 732 cells and increased their rate of apoptosis, whereas the DR 1 antagonist SCH ‐23390 abolished the effects of SKF ‐38393. In OS 732 cells, overexpression of DR 1 increased the rate of apoptosis, caspase‐9 and ‐3 expression, and the release of cytochrome c (Cyt c ), reduced Bcl‐2 expression, inhibited extracellular signal‐regulated kinase 1/2 ( ERK 1/2) phosphorylation, and induced phosphorylation of p38 mitogen‐activated protein kinase (p38 MAPK ) and c‐Jun N‐terminal kinase ( JNK ). These results suggest that activation of DR 1 induces osteosarcoma cell apoptosis via changes to the MAPK pathway.