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Hypertonic saline inhibits airway smooth muscle contraction by inhibiting Ca 2+ sensitization
Author(s) -
Liu XiaoCao,
Wang Qian,
She YuShan,
Chen Shu,
Luo Xi,
Xu Hao,
Zang DunAn,
Zhang WenJing,
Qiu JunYing,
Liu BeiBei,
Shen Jinhua,
Peng YongBo,
Zhao Ping,
Xue Lu,
Chen Weiwei,
Ma LiQun,
Fu Xiangning,
Chen Jingyu,
Liu QingHua,
Yu MengFei
Publication year - 2017
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.12807
Subject(s) - contraction (grammar) , chemistry , acetylcholine , intracellular , muscle contraction , hypertonic saline , myosin light chain phosphatase , myosin light chain kinase , tonicity , sensitization , cytosol , myosin , endocrinology , phosphatase , biophysics , medicine , phosphorylation , biochemistry , biology , enzyme , immunology
Summary The effects of hypertonic solution on airway smooth muscle ( ASM ) contraction and the underlying mechanisms are largely unknown. We found that hypertonic saline ( HS ) inhibited acetylcholine ( AC h)‐induced contraction of ASM from the mouse trachea and human bronchi. In single mouse ASM cells ( ASMC s), AC h induced an increase in intracellular Ca 2+ that was further enhanced by 5% NaCl, indicating that the HS ‐induced inhibition of ASM contraction was not mediated by a decrease in cytosolic Ca 2+ . The Rho‐associated kinase ( ROCK ) inhibitor Y‐27632 relaxed AC h‐induced precontraction of mouse tracheal rings. However, such inhibition was not observed after the relaxation induced by 5% NaCl. Moreover, the incubation of mouse tracheal rings with 5% NaCl decreased AC h‐induced phosphorylation of myosin light chain 20 and myosin phosphatase target subunit 1. These data indicate that HS inhibits the contraction of ASM by inhibiting Ca 2+ sensitization, not by decreasing intracellular Ca 2+ .