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Increased PD ‐L1 expression in breast and colon cancer stem cells
Author(s) -
Wu Yanheng,
Chen Mingshui,
Wu Peihong,
Chen Chen,
Xu Zhi Ping,
Gu Wenyi
Publication year - 2017
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.12732
Subject(s) - stem cell , colorectal cancer , breast cancer , cancer research , cancer stem cell , medicine , cancer , biology , microbiology and biotechnology
Summary Here we report the expression of programmed cell death ligand 1/2 ( PD ‐L1/L2) in breast and colon cancer stem cells ( CSC s). The stemness of these cells was confirmed by their surface markers. Using flow cytometry analysis we demonstrated that PD ‐L1 expression was higher in CSC s of both cancers compared to non‐stem like cancer cells. Consistent with this, detection of cellular PD ‐L1 proteins by western blot assay also showed increased PD ‐L1 protein in CSC s. In contrast, only trace amounts of PD ‐L2 were detected in CSC s of both cancers. Our results suggest that breast and colon cancers may be sensitive to PD 1/ PD ‐L1 immunotherapy and thus warrant further investigations of CSC targeted PD 1/ PD ‐L1 therapy.