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Alleviative effect of ciliary neurotrophic factor analogue on high fat‐induced hepatic steatosis is partially independent of the central regulation
Author(s) -
Cui MingXia,
Yang LiNing,
Wang XiaoXia,
Wang Lei,
Li RuiLian,
Han Wei,
Wu YongJie
Publication year - 2017
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.12709
Subject(s) - ciliary neurotrophic factor , steatosis , medicine , endocrinology , fatty liver , triglyceride , neurotrophic factors , nitric oxide , chemistry , biology , receptor , cholesterol , disease
Summary Ciliary neurotrophic factor ( CNTF ) analogues were reported to ameliorate fatty liver in db/db or high‐fat diet‐fed mice. It is generally thought that CNTF exerts its actions centrally. The aim of this study was to investigate whether peripheral effects of CNTF analogues are involved in the therapeutic effect on high fat‐induced hepatic steatosis. The rat model of fatty liver was induced by a high‐fat diet ( HFD ) for 12 weeks. In the next 2 weeks, rats were fed the HFD along with subcutaneous injection of vehicle or mutant recombinant human CNTF (rhm CNTF 0.05‐0.2 mg/kg per day). Steatotic HepG2 cells were induced by 50% fetal bovine serum ( FBS ) for 48 hours, and then treated with rhm CNTF for 24 hours. The results showed that after rhm CNTF treatment, hepatic triglyceride ( TG ) accumulation was attenuated both in vivo and in vitro. Rhm CNTF increased protein expression of CPT ‐1 and PPAR α, and decreased SREBP ‐1c, FAS and SCD ‐1 in steatotic HepG2 cells. But the production of nitric oxide and 8‐iso PGF 2α in steatotic HepG2 cells was not affected by rhm CNTF . These results suggest that rhm CNTF has a peripheral effect that alleviates fat‐induced hepatic steatosis.