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Effects of high‐sodium intake on systemic blood pressure and vascular responses in spontaneously diabetic WBN /Kob‐ Lepr fa/fa rats
Author(s) -
Takagi Yoshiichi,
Kadowaki Haruno,
Kobayashi Ikumi,
Ito Kaoru,
Ito Katsuaki,
Shirai Mitsuyuki,
Asai Fumitoshi
Publication year - 2017
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.12700
Subject(s) - endocrinology , medicine , sodium nitroprusside , blood pressure , phenylephrine , sodium , prostaglandin , chemistry , nitric oxide , organic chemistry
Summary The prevalence of type 2 diabetes mellitus (T2 DM ) and hypertension has markedly increased worldwide. The purpose of the present study was to examine the effects of a high‐salt intake on the systolic blood pressure ( SBP ) and vascular responses in WBN /Kob‐ Lepr fa/fa ( WBKDF ) rats, a new spontaneous animal model of T2 DM . Male WBKDF rats and age‐matched Wistar rats at 6 weeks of age were each divided into two groups and fed either a normal‐sodium ( NS , 0.26%) diet or high‐sodium ( HS , 8%) diet for 14 weeks: (i) Wistar rats on NS diet (Wistar‐ NS ); (ii) Wistar rats on HS diet (Wistar‐ HS ); (iii) WBKDF rats on NS diet ( WBKDF ‐ NS ); (iv) WBKDF rats on HS diets ( WBKDF ‐ HS ). Neither WBKDF ‐ NS nor Wistar‐ NS rats showed significant changes in SBP throughout the experiment, but both WBKDF ‐ HS and Wistar‐ HS exhibited significant elevation of SBP , which was more prominent ( P <.01) in WBKDF ‐ HS than in Wistar‐ HS . Phenylephrine‐induced contractions of isolated thoracic aortic rings were significantly ( P <.01) enhanced in WBKDF ‐ HS and Wistar‐ HS compared with the respective strain of rats on the NS diet. In contrast, acetylcholine‐ and nitroprusside‐induced relaxation were significantly ( P <.01) diminished in both WBKDF ‐ HS and Wistar‐ HS , and these HS diet‐induced changes were more profound ( P <.01) in WBKDF rats than in Wistar rats. Significantly ( P <.05) higher plasma concentrations of 8‐iso‐prostaglandin F 2α and sodium ions were observed in WBKDF ‐ HS than in Wistar‐ HS . The current study demonstrated that WBKDF ‐ HS rats developed salt‐sensitive hypertension associated with vascular dysfunction. The WBKDF rat may be a useful model for investigating the etiology of hypertension with T2 DM .