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Smart bomb AS 1411 aptamer‐functionalized/ PAMAM dendrimer nanocarriers for targeted drug delivery in the treatment of gastric cancer
Author(s) -
Barzegar Behrooz Amir,
Nabavizadeh Fatemeh,
Adiban Jamal,
Shafiee Ardestani Mehdi,
Vahabpour Rouhollah,
Aghasadeghi Mohammad Reza,
Sohanaki Hamid
Publication year - 2017
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.12670
Subject(s) - dendrimer , nanocarriers , drug delivery , aptamer , polyethylene glycol , peg ratio , conjugated system , chemistry , cancer , drug , materials science , nanotechnology , pharmacology , biophysics , microbiology and biotechnology , medicine , biochemistry , biology , organic chemistry , polymer , finance , economics
Summary Chemotherapy, a conventional method assessed in recent oncology studies, poses numerous problems in the clinical environment. To overcome the problems inherent in chemotherapy, an intelligent drug delivery system has come to the forefront of cancer therapeutics. In this study, we designed a dendrimer‐based pharmaceutical system together with a single‐stranded AS 1411 aptamer ( APT AS 1411 ) as a therapeutic strategy. The polyamidoamine ( PAMAM )‐polyethylene glycol ( PEG ) complex was then conjugated with the AS 1411 aptamer and confirmed by atomic‐force microscopy ( AFM ) and Fourier transform infrared spectroscopy ( FTIR ) .In this study, we show that the conjugated PAMAM ‐ PEG ‐ APT AS 1411 complex dramatically increased PAMAM ‐ PEG ‐5‐ FU uptake by MKN 45 gastric cancer cells. We also demonstrated both the stability of the nanoparticle‐5‐ FU ‐ APT AS 1411 complex, by thin layer chromatography ( TLC ), and an increase in 5‐fluorouracil (5‐ FU ) accumulation in the vicinity of cancerous tumors. This smart drug delivery system is capable of effectively transferring 5‐ FU to MKN 45 gastric cancer cells in consistent and without toxic effects.