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Epigallocatechin‐3‐gallate augments therapeutic effects of mesenchymal stem cells in skin wound healing
Author(s) -
Li Min,
Xu Jingxing,
Shi Tongxin,
Yu Haiyang,
Bi Jianping,
Chen Guanzhi
Publication year - 2016
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.12652
Subject(s) - wound healing , mesenchymal stem cell , inflammation , angiogenesis , pharmacology , medicine , neovascularization , tumor necrosis factor alpha , granulation tissue , skin repair , downregulation and upregulation , cancer research , immunology , chemistry , pathology , biochemistry , gene
Summary In non‐healing wounds, mesenchymal stem cell ( MSC )‐based therapies have the potential to activate a series of coordinated cellular processes, including angiogenesis, inflammation, cell migration, proliferation and epidermal terminal differentiation. As pro‐inflammatory reactions play indispensable roles in initiating wound repair, sustained and prolonged inflammation exhibit detrimental effects on skin wound closure. We investigated the feasibility of using an antioxidant agent epigallocatechin‐3‐gallate ( EGCG ), along with MSC s, to improve wound repair through their immunomodulatory actions. In a rat model of wound healing, a single dose of EGCG at 10 mg/kg increased the efficiency of MSC ‐induced skin wound closure. Twenty days after the wound induction, MSC treatment significantly enhanced the epidermal thickness, which was further increased by EGCG administration. Consistently, the highest extent of growth factors upregulation for neovascularization induction was seen in the animals treated by both MSC s and EGCG , associated with a potent anti‐scarring effect throughout the healing process. Finally, expression levels of pro‐inflammatory cytokines, such as tumor necrosis factor‐α ( TNF ‐α), interleukin‐1β ( IL ‐1β) and IL ‐6, in the wound area were reduced by MSC s, and this reduction was further potentiated by EGCG co‐administration. EGCG , together with MSC s, can promote skin wound healing likely through their combinational effects in modulating chronic inflammation.