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Role of mechanistic target of rapamycin ( mTOR ) in renal function and ischaemia–reperfusion induced kidney injury
Author(s) -
Alshaman Reem,
Truong Luan,
Oyekan Adebayo
Publication year - 2016
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.12648
Subject(s) - pi3k/akt/mtor pathway , creatinine , renal function , autophagy , sirolimus , kidney , endocrinology , medicine , chemistry , excretion , acute kidney injury , pharmacology , apoptosis , biochemistry
Summary Despite the presence of many studies on the role of mechanistic target of rapamycin ( mTOR ) in cardiorenal tissues, the definitive role of mTOR in the pathogenesis of renal injury subsequent to ischaemia–reperfusion ( IR ) remains unclear. The aims of the current study were to characterize the role of mTOR in normal kidney function and to investigate the role of mTOR activation in IR ‐induced kidney injury. In euvolemic anaesthetized rats, treatment with the mTOR inhibitor rapamycin increased blood pressure (121 ± 2 to 144 ± 3 mmHg; P <.05), decreased glomerular filtration rate ( GFR ; 1.6 ± 0.3 to 0.5 ± 0.2 mL /min; P <.05) and increased urinary sodium excretion ( UN aV; 14 ± 1 to 109 ± 25 mmol/L per hour; P <.05). In rats subjected to IR , autophagy induction, p‐ mTOR expression and serum creatinine increased (1.9 ± 0.2 to 3 ± 0.3 mg/ dL ; P <.05); treatment with rapamycin blunted p‐ mTOR expression but further increased autophagy induction and serum creatinine (3 ± 0.3 to 5 ± 0.6 mg/ dL ; P <.05). In contrast, clenbuterol, an mTOR activator, blunted the effect of rapamycin on serum creatinine (4 ± 0.6 vs 2.3 ± 0.3 mg/ dL ; P <.05), autophagy induction and p‐ mTOR expression. IR also increased 24 hour protein excretion (9 ± 3 to 17 ± 2 mg/day; P <.05) and kidney injury molecule‐1 ( KIM ‐1) expression, and rapamycin treatment further increased KIM ‐1 expression. Clenbuterol exacerbated protein excretion (13 ± 2 to 26 ± 4 mg/day; P <.05) and antagonized the effect of rapamycin on KIM ‐1 expression. Histopathological data demonstrated kidney injury in IR rats that was worsened by rapamycin treatment but attenuated by clenbuterol treatment. Thus, mTOR signalling is crucial for normal kidney function and protecting the kidney against IR injury through autophagy suppression.