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Protection of W istar‐ F urth rats against postischaemic acute renal injury: Role for nitric oxide and thromboxane?
Author(s) -
Voisin Viginie,
Declèves AnneEmilie,
Hubert Virginie,
Colombaro Vanessa,
Giordano Laetitia,
Habsch Isabelle,
Bouby Nadine,
clercq Denis,
Caron Nathalie
Publication year - 2014
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.12298
Subject(s) - medicine , thromboxane , endocrinology , kidney , transplantation , renal function , nitric oxide synthase , nitric oxide , inflammation , nephrectomy , chemistry , platelet
Summary The Wistar‐Furth ( WF ) rat strain is usually used in models of full major histocompatibility complex‐mismatched kidney transplantation. Because these rats have been demonstrated to be resistant to several models of chronic kidney disease, the aim of the present study was to investigate their potential resistance to renal ischaemia–reperfusion (I/R) injury compared with another strain, namely Wistar‐Hanover ( WH ) rats. Anaesthetized male WH and WF rats were submitted to I/R by occlusion of the left renal artery and contralateral nephrectomy. Urine, blood and tissue samples were collected at different time points after I/R to evaluate renal function, inflammation and tubular injury, along with determination of nitric oxide synthase ( NOS ) expression and thromboxane A 2 (TxA 2 ) production. Post‐ischaemic renal function was better preserved in WF than WH rats, as evidenced by reduced levels of creatininaemia, urinary neutrophil gelatinase‐associated lipocalin excretion and proteinuria. In addition, WF rats had less intrarenal inflammation than WH rats after I/R injury. These observations were associated with maintenance of neuronal NOS expression, along with lower induction of inducible NOS expression in WF versus WH rats. Moreover, WF rats excreted a significantly lower amount of TxB 2 . The results indicate that WF rats are more resistant to an I/R injury than WH rats in terms of renal function and inflammation. These observations are associated with differential regulation of intrarenal NOS expression, as well as a reduction in thromboxane production, which could contribute to a better outcome for the postischaemic kidney in WF rats.