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p27 kip1 deficiency accelerates dentin and alveolar bone formation
Author(s) -
Yin Ying,
Wang Qun,
Sun Wen,
Wang Yuli,
Chen Ning,
Miao Dengshun
Publication year - 2014
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.12276
Subject(s) - chemistry , bone sialoprotein , dental alveolus , dentin , osteoblast , endocrinology , alkaline phosphatase , medicine , osteocalcin , type i collagen , runx2 , bone morphogenetic protein 2 , anatomy , dentistry , biology , biochemistry , in vitro , enzyme
Summary To assess the role of p27 kip1 in regulating dental formation and alveolar bone development, we compared the teeth and mandible phenotypes of homozygous p27 kip1 ‐deficient (p27 −/− ) mice with their wild‐type littermates at 2 weeks of age. At 2 weeks of age, dental mineral density, dental volume and dentin sialoprotein‐immunopositive areas were increased significantly, whereas the predentin area : total dentin area and biglycan‐immunopositive area : dentin area ratios were decreased significantly in p27 −/− mice compared with their wild‐type (WT) littermates. Mandible mineral density, cortical thickness, alveolar bone volume, type I collagen and osterix‐immunopositive areas, osteoblast number and activity and mRNA expression of Runt‐related transcription factor 2 ( Runx2 ), alkaline phosphatase ( ALP ), osteocalcin and bone morphogenetic protein ( bmp2 ) were all significantly increased in the mandibles, as was the number and surface of tartrate‐resistant acid phosphatase‐positive osteoclasts in the alveolar bone of p27 −/− mice compared with their WT littermates. Furthermore, the percentage of proliferating cell nuclear antigen‐positive cells in Hertwig's epithelial root sheath and protein expression of cyclin E and cyclin‐dependent kinase 2 were increased significantly in p27 −/− mice relative to their WT littermates. The results from this study indicate that p27 plays a negative regulatory role in dentin formation and alveolar bone development.