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Kidney regeneration by non‐platelet RNA ‐containing particle‐derived cells
Author(s) -
Kong Wuyi,
Nuo Mu,
Zhu Xiao Ping,
Han Xiu Juan,
Wang Xian
Publication year - 2013
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.12164
Subject(s) - regeneration (biology) , platelet , kidney , rna , microbiology and biotechnology , particle (ecology) , chemistry , biology , medicine , endocrinology , biochemistry , ecology , gene
Summary We found a group of non‐platelet RNA ‐containing particles ( NPRCP s) in human umbilical cord blood. These particles can aggregate, fuse and become non‐nucleated cells when cocultured with nucleated cells in vitro . The non‐nucleated cells further differentiate into nucleated cells expressing octamer binding transcription factor 4 ( OCT 4). The NPRCP s are approximately 1–5 μm in diameter, have a thin bilayer membrane, contain short RNA s and micro RNA s and express OCT 4, sex‐determining region Y 2 ( SOX 2) and DEAD box polypeptide 4 ( DDX 4). To confirm the function of NPRCP s in vivo , we examined the effects of tail vein‐injected green fluorescent protein ( GFP )‐labelled NPRCP s on mouse kidneys damaged by prior ischaemia and reperfusion from Day 1 to Week 6. Within 1 day of injection of NPRCP s, immunofluorescence and immunohistochemistry revealed a large number of extravasated NPRCP s in the renal calyces, damaged glomeruli and duct tubules. During the course of regeneration, NPRCP s fused into large, non‐nucleated cellular structures that further became large nucleated cells to regenerate multicellular kidney tubules. In addition, many NPRCP s became tiny nucleated cellular structures that further differentiated into interstitial cells in connective tissue. The extravasated NPRCP s also arranged themselves into non‐cell glomerular structures before further regenerating into nucleated cells of the glomerulus. In conclusion, the results demonstrate that, via different patterns of differentiation, NPRCP ‐derived cells can regenerate mouse kidney tissue damaged by ischaemia.