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Growth receptor binding protein 10 inhibits glucose‐stimulated insulin release from pancreatic β‐cells associated with suppression of the insulin/insulin‐like growth factor‐1 signalling pathway
Author(s) -
Li Ling,
Li Xiaowen,
Zhu Yunxia,
Zhang Mingliang,
Yin Dechao,
Lu Junxi,
Liu Feng,
Wang Chen,
Jia Weiping
Publication year - 2013
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.12160
Subject(s) - medicine , insulin , endocrinology , growth factor , receptor , insulin receptor , insulin like growth factor , insulin receptor substrate , chemistry , grb10 , biology , insulin resistance
Summary Growth receptor binding protein 10 (Grb10) is an adaptor protein that interacts with the insulin receptor and insulin‐like growth factor ( IGF )‐1 receptor. Overexpression of Grb10 in muscle cells and adipocytes inhibits insulin signalling, and transgenic mice overexpressing Grb10 exhibit impaired glucose tolerance. However, the roles of Grb10 in β‐cells remain unknown. The aim of the present study was to explore the effect of Grb10 on β‐cell function. The effects of Grb10 on glucose‐stimulated insulin secretion ( GSIS ) and the insulin/ IGF ‐1 signalling pathway were investigated in rat islets and/or dispersed islet cells with Grb10 overexpresion by adenovirus transfection. Protein expression was detected by western blot analysis. We found that Grb10 was expressed in both human and rat pancreas. Expression of Grb10 was increased in islets isolated from rats fed a high‐fat plus high‐sugar diet compared with islets isolated from rats fed normal chow diet, as well as in INS 832/13 cells exposed to high levels of glucose (20 mmol/L), palmitate (1 mmol/L) and interleukin‐1β (50 U/mL). Overexpression of Grb10 in INS 832/13 cells or rat islets impaired GSIS compared with the respective control (all P < 0.05). Moreover, inhibition of GSIS by Grb10 overexpression was associated with a decrease in insulin‐ and IGF ‐1‐induced Akt and extracellular signal‐regulated kinase 1/2 phosphorylation. The results of the present study demonstrate that Grb10 is an important negative regulator of insulin/ IGF ‐1 signalling in pancreatic β‐cells and a potential target to improve β‐cell function.