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Multiscale measurement of cardiac energetics
Author(s) -
Goo Soyeon,
Pham Toan,
Han JunChiew,
Nielsen Poul,
Taberner Andrew,
Hickey Anthony,
Loiselle Denis
Publication year - 2013
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/1440-1681.12139
Subject(s) - energetics , oxygen , yield (engineering) , chemistry , work (physics) , biophysics , materials science , thermodynamics , physics , biology , organic chemistry
Summary Herein we describe our laboratories' experimental methods for interrogating cardiac energetics at the organ (whole heart), tissue (trabecula) and perforated fibre (mitochondrial) levels. In whole heart and trabecula experiments, we focus on measuring pressure–volume (force–length) work and oxygen consumption (heat production) from which mechanical efficiency is derived. In both preparations (i.e. across scales differing by three orders of magnitude) we find efficiency values of 10%–15%. Mitochondrial experiments invoke a trio of titration protocols to yield information on oxygen consumption, ATP flux, membrane potential, electron leak and reactive oxygen species production, the latter two of which index energy transfer inefficiencies.