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Abscisic‐acid‐induced turion formation in Spirodela polyrrhiza L. IV . Comparative ion flux characteristics of the turion and the vegetative frond and the effect of ABA during early turion development
Author(s) -
SMART CHERYL C.,
TREWAVAS ANTHONY J.
Publication year - 1984
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/1365-3040.ep11616215
Subject(s) - frond , abscisic acid , vacuole , cytoplasm , botany , biology , chemistry , biophysics , biochemistry , gene
Using the method of compartmental analysis, the ion fluxes and compartment concentrations of Ca 2+ , K + and Cl ‐ have been compared in the untreated vegetative frond and the abscisic acid (ABA) induced turion of Spirodela polyrrhiza. The ABA‐induced turion is characterized by reduced Ca 2+ exchange across the tonoplast and low vacuolar Ca 2+ concentration relative to the vegetative frond. In addition the turion exhibits a higher plasmalemma flux with a correspondingly high Ca 2+ concentration in the cytoplasm. The concentration of K + and Cl ‐ is much lower in the cytoplasm of the ABA‐induced turion than in the vegetative frond with the influx/efflux ratio at both the plasmalemma and the tonoplast being less than 1, a finding exhibited also in dormant storage tissue. Treatment of vegetative fronds with ABA for 18 h resulted in a reduced K + plasmalemma efflux relative to untreated vegetative fronds and a concomitant increase in the cytoplasmic concentration. There was no rapid effect of ABA on Ca 2+ , K + or Cl ‐ fluxes through either membrane. These results are consistent with the notion that drastic changes in ion fluxes and concentrations in the turion are a secondary consequence of ABA‐induced development, possibly due to prior regulation by ABA of enzymes inherent to processes involved in membrane transport.