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Differential Na 2 SO 4 tolerance in tobacco plants regenerated from Na 2 SO 4 ‐grown callus
Author(s) -
PUA ENGCHONG,
TREVOR A.
Publication year - 1986
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/1365-3040.ep11612678
Subject(s) - shoot , callus , seedling , germination , acclimatization , nicotiana tabacum , horticulture , biology , dry weight , botany , biochemistry , gene
The regenerated shoots from sodium sulphate (Na 2 SO 4 ) grown callus of tobacco ( Nicotiana tabacum L. cv. Wisconsin 38) were evaluated for Na 2 SO 4 tolerance based on shoot proliferation and rooting in vitro , and seed germination in vivo in response to Na 2 SO 4 . An increase in Na 2 SO 4 concentration resulted in significantly decreasing shoot fresh weight, number of shoots, shoot length and leaf size, and increasing per cent shoot dry weight of both control and Na 2 SO 4 ‐grown cultures. In rooting, shoots of Na 2 SO 4 ‐grown cultures exhibited the highest per cent rooting (85%) in the presence of 1% w/v Na 2 SO 4 . However, per cent rooting, root number per rooted cutting and root fresh weight decreased significantly with increasing Na 2 SO 4 concentration when shoots were transferred to the medium in the absence of Na 2 SO 4 for 4‐monthly passages. Following acclimatization of the rooted shoots of Na 2 SO 4 ‐grown cultures, phenotypic variation was observed during growth and development. There were 13.2% sterile plants. Fertile plants were sorted into normal (N), tolerant (T), and sensitive (S) categories and the respective percentages of plants were 31.6, 44.7 and 10.5, based on per cent germination, germination velocity index and seedling survival to Na 2 SO 4 . The response of N, T and S types to Na 2 SO 4 in subsequent shoot proliferation was similar to that of seed germination.