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The effect of short‐term H 2 S fumigation on water‐soluble sulphydryl and glutathione levels in spinach
Author(s) -
KOK L. J.,
BOSMA W.,
MAAS F. M.,
KUIPER P. J. C.
Publication year - 1985
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/1365-3040.ep11604605
Subject(s) - fumigation , glutathione , spinacia , spinach , shoot , chemistry , horticulture , botany , biochemistry , biology , chloroplast , gene , enzyme
. Short‐term fumigation of Spinacia oleracea with 380 μg m −3 H 2 S (250 ppb) resulted in a rapid accumulation of water‐soluble SH‐compounds in the shoots. After 1 h exposure a substantial increase in the SH‐content was already detectable and maximal accumulation, three‐ to four‐fold that in control plants, was observed after 24 h of exposure. Irradiation during H 2 S exposure only slightly affected the rate and level of SH‐accumulation. H 2 S fumigation did not affect the water‐soluble SH‐content of the roots. Glutathione was the sole water‐soluble SH‐compound accumulating upon exposure to H 2 S. It was calculated that during the first hour of exposure to 380 μg m −3 H 2 S 39% of the possible absorbed H 2 S was converted into glutathione. The SH‐content of the water‐soluble proteins of the shoots was not affected by H 2 S exposure. When fumigation was stopped, a rapid decrease in glutathione content was observed and after 48 h the content was comparable to that of the control plants. Contrary to H 2 S, SO 2 fumigation did not result in a rapid accumulation of glutathione in spinach shoots. The possible role of glutathione accumulation during H 2 S fumigation is discussed.