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Methods for quantitative determination of ethylene in aqueous solutions for biological studies
Author(s) -
BASSI P. K.,
EASTWELL K. C.,
AKALEHIYWOT T.,
SPENCER MARY S.
Publication year - 1981
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/1365-3040.ep11604542
Subject(s) - ethylene , stripping (fiber) , aqueous solution , extraction (chemistry) , chromatography , chemistry , gas chromatography , quantitative analysis (chemistry) , continuous flow , materials science , analytical chemistry (journal) , organic chemistry , biochemical engineering , engineering , composite material , catalysis
. In many plant physiological studies, quantitative determination of ethylene in aqueous solutions is necessary. We describe two methods for such measurements based on vacuum extraction, or on stripping ethylene from solution with the carrier gas of a gas chromatograph. The gas‐stripping technique is limited by the small sample volumes that can be used, whereas the vacuum extraction technique can accommodate much larger volumes and is useful when the ethylene concentration in the solution is very low. The use of these techniques will facilitate the treatment of submerged plant tissues with known amounts of ethylene, and also the quantitative determination of ethylene from tissues grown in either static or continuous flow liquid cultures.