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Mycochromone decreases ATP‐driven proton translocation in plant plasma membrane‐ and tonoplast‐enriched vesicles
Author(s) -
MACRI F.,
VIANELLO A.,
COCUCCI M. C.
Publication year - 1987
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/1365-3040.ep11603612
Subject(s) - nigericin , vesicle , vacuole , electrochemical gradient , atpase , biophysics , biochemistry , proton transport , atp synthase , chemistry , atp hydrolysis , chromosomal translocation , metabolite , f atpase , membrane , biology , chloroplast , cytoplasm , enzyme , thylakoid , gene
Mycochromone, a metabolite produced by Mycosphaerella rosigena , inhibits the ATP‐dependent proton translocation and the ATP‐generated electrical potential in pea stem tonoplast‐enriched vesicles, without affecting the H + /K + exchange induced by nigericin or an artificially imposed proton gradient. The inhibition is dependent on the time of pre‐incubation and mycochromone concentration. In addition, mycochromone inhibits the ATP‐dependent proton translocation in radish plasma membrane‐enriched vesicles, though it does not alter ATPase activity (evaluated by hydrolysis of ATP) in either type of plant vesicle. Mycochromone seems to act on the H + channels for proton translocation of the H + ‐pumping ATPase localized on plasmalemma and tonoplast, without affecting the catalytic site of ATP hydrolysis.