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Movement of fluorescein into isolated caryopses of wheat and barley
Author(s) -
COOK H.,
OPARKA K. J.
Publication year - 1983
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/1365-3040.ep11587641
Subject(s) - endosperm , caryopsis , phloem , apoplast , xylem , biology , fluorescein , biophysics , botany , fluorescence , poaceae , cell wall , optics , physics
. The movement of fluorescein, a symplastic fluorescent tracer, into isolated caryopses of wheat and barley is described. The dye followed the pathway to the endosperm which has been proposed previously from anatomical studies, namely a movement from the phloem, through cells of the pigment strand and nucellar projection, followed by a radial spread of the dye from the endosperm cavity into the starchy endosperm. By contrast, the fluorochromes calcofluor white M2R and ANS remained confined to the apoplast and failed to cross the ‘xylem discontinuity’ at the base of the caryopses.