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Absence of nitrate reductase activity in San 9789 bleached leaves of barley seedlings ( Hordeum vulgare cv. Midas)
Author(s) -
DEANEDRUMMOND C. E.,
JOHNSON C. B.
Publication year - 1980
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/1365-3040.ep11581851
Subject(s) - nitrate reductase , hordeum vulgare , nitrate , photosynthesis , chlorophyll , chloroplast , nitrite reductase , pigment , phytochrome , chemistry , carotenoid , reductase , botany , hordeum , horticulture , biology , poaceae , enzyme , biochemistry , red light , organic chemistry , gene
San 9789 (norflurazone) blocks carotenoid synthesis which allows chlorophyll bleaching in the light, and has been used recently as a tool to study phytochrome responses without interference from photosynthetic pigments. By using this herbicide, we have found that nitrate reductase activity and light dependent nitrite reduction were lost simultaneously from achlorophyllous areas of barley leaves, with the green areas of the leaf tip still showing high activities. By contrast nitrate reductase is still present in the roots of herbicide treated plants. We suggest that intact chloroplasts are required for the presence of nitrate reductase in barley leaves.